The HESI Biology test consists of 25 multiple choice questions which must be answered within 25 minutes. Questions will cover your knowledge of basic biology, including biologic molecules, water, cellular energy production, cellular reproduction, DNA, metabolism, and genetics. Our free HESI biology practice test includes 50 questions to assist with your test prep and review.
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Question 1 of 50
1. Question
Proteins are polymers composed of
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Question 2 of 50
2. Question
What type of bond links amino acids together within the primary structure of a protein?
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Question 3 of 50
3. Question
Which nucleotide base is found within DNA but not in RNA?
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Question 4 of 50
4. Question
Glucose is a common monomer of
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Question 5 of 50
5. Question
What is the type of bond between the O atom and each H atom within a water molecule called?
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Question 6 of 50
6. Question
What property allows refers to the ability of water molecules to stick to each other?
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Question 7 of 50
7. Question
Which statement describes the relationship between the densities of ice and liquid water?
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Question 8 of 50
8. Question
What type of bond forms between water molecules that allows them to stick to each other?
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Question 9 of 50
9. Question
Which cellular structure is found in a typical eukaryote but not in a prokaryote?
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Question 10 of 50
10. Question
Which step of the scientific method occurs before the development of a hypothesis?
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Question 11 of 50
11. Question
Which of the following is an example of an anabolic pathway?
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Question 12 of 50
12. Question
What type of molecule are hormones like testosterone and estrogen?
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Question 13 of 50
13. Question
What organelle of a eukaryotic cell is important for modifying proteins and then packaging them into membrane-bound vesicles for transport and secretion?
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Question 14 of 50
14. Question
Which eukaryotic organelle digests foreign materials like viruses by phagocytosis?
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Question 15 of 50
15. Question
Which statement best describes the hydrophobic part of a cell membrane?
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Question 16 of 50
16. Question
Which organelle likely arose as a result of the endosymbiosis of a bacterium inside of a primitive eukaryotic cell?
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Question 17 of 50
17. Question
Which molecule passes through the cell membrane by simple diffusion?
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Question 18 of 50
18. Question
During cellular respiration, which process results in the degradation of acetyl-CoA to CO2?
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Question 19 of 50
19. Question
How many net ATP molecules are made per glucose molecule during glycolysis?
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Question 20 of 50
20. Question
Where is the electron transport chain located in a eukaryotic cell?
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Question 21 of 50
21. Question
Which of the following is a product of photosynthesis?
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Question 22 of 50
22. Question
Which type of organism is a prokaryote?
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Question 23 of 50
23. Question
Which level of biological organization is the smallest unit of structure that is considered to be alive?
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Question 24 of 50
24. Question
Which level of biological organization is the smallest unit that can evolve?
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Question 25 of 50
25. Question
Which list has the levels of biological organization correctly ordered from smallest to largest?
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Question 26 of 50
26. Question
During which photosynthetic process is CO2 fixed into organic carbon?
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Question 27 of 50
27. Question
During which phase of the eukaryotic cell cycle does DNA replication occur?
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Question 28 of 50
28. Question
Consider the cell cycle. If a cell has 10 picograms of DNA during G2 phase, how much DNA does it have during G1 phase?
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Question 29 of 50
29. Question
During which stage of meiosis does crossing over occur?
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Question 30 of 50
30. Question
During which stage of meiosis does the separation of homologous chromosome pairs occur?
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Question 31 of 50
31. Question
Which process do prokaryotes use for cell division?
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Question 32 of 50
32. Question
Consider a cell undergoing meiosis. Which best describes the cells resulting from meiosis I?
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Question 33 of 50
33. Question
How many pairs of autosomes do humans have?
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Question 34 of 50
34. Question
If an individual heterozygous for a gene mates with an individual who is homozygous recessive for the same gene, what is the probability that their offspring will be homozygous recessive?
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Question 35 of 50
35. Question
If the A allele is completely dominant to the a allele, which groups of genotypes will result in an individual having the dominant phenotype?
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Question 36 of 50
36. Question
Cystic fibrosis is an autosomal recessive disease. If two individuals who do not suffer from cystic fibrosis but each carry the allele associated with the disease have children, what is the probability that they will have a child having cystic fibrosis?
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Question 37 of 50
37. Question
Which term refers to an individual’s observable characteristics resulting from what genes it has as well as environmental influences?
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Question 38 of 50
38. Question
If two individuals both having the genotype AaBb are crossed, what proportion of their offspring do you expect to have the dominant A phenotype combined with the recessive b phenotype?
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Question 39 of 50
39. Question
What term refers to 2 chromosomes that have the same genes in the same order but not necessarily the same alleles of each gene?
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Question 40 of 50
40. Question
Within a double-stranded DNA molecule, what type of bond links nucleotides within a strand of DNA to each other?
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Question 41 of 50
41. Question
Within a double-stranded molecule of DNA, if 20% of the nucleotides contain A, what percentage of nucleotides contain C?
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Question 42 of 50
42. Question
Where does transcription occur in a eukaryotic cell?
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Question 43 of 50
43. Question
Which of the following is the chemical formula for a carbohydrate composed of 12 carbons?
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Question 44 of 50
44. Question
Which type of atom is found in both proteins and nucleic acids, but not in fats or carbohydrates?
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Question 45 of 50
45. Question
Which of the following is a DNA sequence that signals the initiation of transcription?
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Question 46 of 50
46. Question
Which of the following molecules is involved in transcription but not in translation?
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Question 47 of 50
47. Question
Which level of protein structure refers to the association of the various subunits of a multi-subunited protein into a three-dimensional functional protein?
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Question 48 of 50
48. Question
Which molecule contains 3-nucleotide groupings called codons which each code for a specific amino acid?
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Question 49 of 50
49. Question
Transcribe the following DNA template: 3′-TACGGTACCATG-5′
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Question 50 of 50
50. Question
Which structure or enzyme complex is responsible for linking together nucleotides during the process of replication?
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